Glycosyltransferases

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Effector Glycosyltransferases in Legionella

Legionella causes severe pneumonia in humans. The pathogen produces an array of effectors, which interfere with host cell functions. Among them are the glucosyltransferases Lgt1, Lgt2 and Lgt3 from L. pneumophila. Lgt1 and Lgt2 are produced predominately in the post-exponential phase of bacterial growth, while synthesis of Lgt3 is induced mainly in the lag-phase before intracellular replication...

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Glycosyltransferases encoded by viruses.

Studies of cellular biology in recent decades have highlighted the crucial roles of glycans in numerous important biological processes, raising the concept of glycomics that is now considered as important as genomics, transcriptomics and proteomics. For millions of years, viruses have been co-evolving with their hosts. Consequently, during this co-evolution process, viruses have acquired mechan...

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Human Blood Group Glycosyltransferases

An N-acetylgalactosaminyltransferase, which converts blood group 0 red blood cells to A cells, was purified to homogeneity from plasma of blood group A, subjects. The enzyme was adsorbed on Sepharose QB, and after washing out the impurities, the enzyme was eluted with UDP. This procedure resulted in a 70,000to lOO,OOO-fold increase in specific activity with recovery of about 80%. Further purifi...

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Comparative aspects of glycosyltransferases.

Glycosyltransferases, the enzymes that build oligosaccharides and glycoconjugates, have received much interest in recent years owing to their biological functions and their potential uses in biotechnology. Despite the fact that many glycosyltransferases recognize similar donor or acceptor substrates, there is surprisingly limited sequence identity between different classes. On the one hand, the...

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Membrane interactions of glycosyltransferases

Many important biological processes occur near or in membranes. The role of membranes is not merely confined to compartmentalization, they also form the matrix for membrane associated proteins and are of functional importance. Membrane associated proteins on the other hand require specific membrane properties for proper function. The interactions between membranes and proteins are thus of param...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1989

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(19)84610-0